Search off-target via BLAST
1) Select database:
Human transcripts (206,723 entries)
2) Determine size of oligos:
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3-1) Paste your data :
The length of sequence should be below 200.
Select an example
Example1 (50bp sequence)
Example2 (100bp sequence)
Example3 (150bp sequence)
Example4 (200bp sequence)
3-2) Load your sequence:
The length of sequence should be less than 200.